Kc Junior Software (biotek) __TOP__
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Protein content was evaluated using the method of Bradford11. Sample absorbance was measured at 595 nm in a microplate reader (Power Wave XS UV-Biotek, KC Junior software, USA) and bovine serum albumin was used as the reference standard. Values were expressed in micrograms of albumin per milligram of lysate (mg/mg).
The concentration of prostaglandin E2 in the cell-free supernatants was measured using a commercial PGE2 enzyme immunoassay kit (Cayman Chemical, USA) following the manufacturer's instructions. The sensitivity of the PGE2 assay was 4 pg/ml and the intra- and interassay coefficients of variation were 7.7 and 10.1 %, respectively. The reading of the reaction in each well was made at an optical density of 405 nm in the ELISA reader (ELx800, BioTek) and using the computer program KC Junior software (Biotek).
Values are expressed as the mean ± SEM of at least three biological replicates in each treatment. Statistical analysis was performed using the GraphPad Prism 5.0 software (San Diego, CA, USA). Data were analysed by one-way analysis of variance (ANOVA) followed by Bonferroni's Multiple Comparison test (multiple group comparisons) or by the Student's t-test (two-group comparisons). Differences were considered significant at p < 0.05.
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