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We considered 11 viruses similar to SARS-CoV-2. These viruses include influenza A viruses including A-H1N132, A-H5N133, and A-H7N934, chronic hepatitis C virus (HCV)35, human immunodeficiency virus type 1 (HIV-1)36, human immunodeficiency virus type 2 (HIV-2)37, hendra virus38, human cytomegalovirus39, MERS-CoV40, respiratory syncytial virus41 and SARS-CoV42. The complete genome sequences of these viruses are downloaded from the NCBI database43. We used MAFFT44 ( , version 7, open source license: GPL or BSD), a multiple sequence alignment tool, to compute virus-virus sequence similarity matrix \({S}_{v}\). All parameters were set as the default values provided by MAFFT.
The DrugVirus.info database49 ( ) provided various VDA-related resources. We obtained 770 VDAs from 69 viruses and 128 drugs after removing the viruses whose complete genome sequences are unknown from the database. The chemical structure of drugs and the complete genome sequences of viruses were downloaded from the DrugBank database and the NCBI database, respectively. Similar to dataset 1, we used RDKit and MAFFT to calculate drug similarity and virus similarity.
Molecular docking technique was applied to compute the intermolecular binding abilities between the predicted anti-SARS-CoV-2 drugs and the SARS-CoV-2 spike protein/human ACE2. The chemical structures of drugs were downloaded in the form of the PDB format files from the DrugBank database. We used AutoDockTools to convert these PDB files into pdbqt files needed by AutoDock4. The structures of SARS-CoV-2 spike receptor-binding domain bound with ACE2 (PDB ID: 6M0J) were downloaded from the RCSB Protein Data Bank51. The spike protein and ACE2 were used as receptors, and the predicted anti-SARS-CoV-2 drugs were used as ligands for the molecular docking.
L.-H.P. and L.S. contributed equally to this work. L.-H.P., J.-L.X., J.-L.Y., and L.-Q.Z. conceived the study, designed the schedule, and analyzed the data. F.-X.L. screened the viruses similar to SARS-CoV-2, X.-F.T. downloaded the genome sequences of viruses and computed virus similarity matrix, L.S. computed drug similarity matrix, L.S., X.-F.T., F.-X.L., and J.-J.W. constructed VDA network, L.S. run random walk algorithm, L.-H.P., G.T., and L.-Q.Z. wrote the paper, J.-L.Y. revised the original draft. All authors read and approved the final manuscript.
A: When Amazon WorkSpaces are provisioned, users receive an email providing instructions on where to download the WorkSpaces clients they need, and how to connect to their WorkSpace. If you are not integrating with an existing Active Directory, the user will have the ability to set a password the first time they attempt to connect to their WorkSpace. If the AWS Directory Services AD Connector has been used to integrate with an existing Active Directory domain, users will use their regular Active Directory credentials to log in.
A: A user needs to have an Amazon WorkSpace provisioned for them, and a broadband Internet connection. To use an Amazon WorkSpaces client application to access their WorkSpace, they will need a supported client device (PC, Mac, Linux, iPad, Android tablet, or Android-compatible Chrome OS device), and an Internet connection with opened TCP ports 443 & either 4172 for PCoIP or 4195 for WSP, and UDP port 4172 for PCoIP or 4195 for WSP.
A: Amazon WorkSpaces bundles that provide the Windows 10 desktop experience currently support English (US), French (Canadian), Korean, and Japanese. You can also download and install language packs for Windows directly from Microsoft. For more information, visit this page. Amazon WorkSpaces client applications currently support English (US), German, Chinese (Simplified), Japanese, French (Canadian), Korean, and Portuguese.
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Q: If I already have a WSP WorkSpace, how do I update it? A: You need to reboot your WorkSpaces instance in order to update the WSP host agent. Also, download and install the latest client. You must update both host agent and client to get the latest performance improvements and features. WSP will fall back to using an older version if either client or host agent is not updated.
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